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| |
Linker
|
Sequence
|
II of the fusion protein
|
Aliphatic Index
|
Gravy
|
|---|
|
Flexible linkers
|
LF1
|
(G8) GGGGGGGG
|
35.08
|
74.92
|
−0.455
|
|
LF2
|
(G4S) GGGGS
|
32.45
|
76.15
|
−0.459
|
|
LF3
|
(G4S)2 GGGGSGGGGS
|
33.82
|
74.12
|
−0.459
|
|
LF4
|
GSAGSAAGSGEF
|
29.26
|
74.92
|
−0.425
|
|
LF5
|
KESGSVSSEQLAQFRSLD
|
34.65
|
77.08
|
−0.487
|
|
LF6
|
EGKSSGSGSESKST
|
32.26
|
72.57
|
−0.537
|
|
Rigid Linkers
|
LR1
|
EAAAK
|
31.55
|
77.80
|
−0.456
|
|
LR2
|
A (EAAAK)2AA
|
30.06
|
77.68
|
−0.419
|
|
LR3
|
(XP)n = APAPAP
|
35.88
|
77.38
|
−0.440
|
|
LR4
|
(XP)n = EPEPEP
|
35.63
|
75.74
|
.527
|
|
LR5
|
(XP)n:KPKPKP
|
28.63
|
75.74
|
−0.533
|
|
LR6
|
(XP)n: GPGP
|
30.32
|
76.57
|
−0.470
|
